Custom qpcr and dpcr pbes microsynth taqman probes

CUSTOM QPCR AND DPCR PBES MICROSYNTH TAQMAN PROBES FULL

CUSTOM QPCR AND DPCR PBES MICROSYNTH TAQMAN PROBES FULL

In the present study, a GATA-like protein (named as EsGLP) was characterized from Eriocheir sinensis, including an 834 bp full length open reading frame of EsGLP, encoding a polypeptide of 277 amino acids. GATA transcription factor is a family of DNA-binding proteins that can recognize and bind to sequence of (A/T) GATA (A/G). Sex determination in pearl oyster is therefore an essential mechanism to understand. Nacre formation has also been reported to be associated with ovarian development. Pearls from males have uniform thickness and smoothness whiles those from females have several scratches. In pearl oysters, gender has been reported to affect nacre formation and pearl quality. Factors such as hormone administration, temperature, pollution, and culture conditions affect sex determination, and sometimes cause sex reversal of pearl oysters. sox 9, foxl 2, Gata-type zinc finger protein I (zglp1), Protein ovo (ovo) and the dmrt genes are involved in genetic sex determination of pearl oysters. Generally, the sex of an organism is determined by two factors either genetic or environmental. The success of these controlled reproduction and the nuclei insertion operation is highly dependent on the state of the gonads and mainly gametogenesis. The commercially utilized method for the production of pearls is nucleus insertion operation, also known as the grafting operation. This has attracted growth and reproduction research being conducted on the pearl oyster, with research targeted at producing viable stocks through breeding programs utilizing controlled reproduction. Pearl aquaculture is an intensive pearl production method which has gained much interest over the years with pearl oysters been the main organism involved in the production of sea pearls. lacustris and provides a valuable dataset for future research on the genes involved in reproduction of G. This work represents the first gonadal transcriptomic analysis of G. Using GO and KEGG enrichment analyses, pathways involving regulation of gonadal development and gametogenesis also identified. Numerous DEGs involved in gonadal development and gametogenesis were identified, including foxl2, dmrt1, cyp19a1a, inha, inhb, sycp2, zglp1, tdrp, zps and esra. RT-qPCR analysis of 14 selected genes showed patterns consistent with the transcriptome results. Of these genes, 2,383 were up-regulated in the testis and 8,571 were up-regulated in the ovary. Gene expression patterns in the testis and ovary were compared, and 10,954 DEGs (differentially expressed genes) were identified. In addition, 38,550 SSRs (simple sequence repeat, microsatellite) were identified from 20,517 SSR containing sequences. A total of 47,891 (76.53%) unigenes were annotated in at least one of the seven databases that were used in this study. Reads were assembled into 62,573 unigenes with N50 value of 3,082 bp and a mean length of 1,869 bp. In total, 60,657,644 and 52,016,136 clean reads were obtained from ovary and testis, respectively, using Illumina sequencing. lacustris for the first time and identified genes that may be involved in gonadal development and reproduction. Here, we constructed gonadal transcriptomes of G. To date, there are no genomic or transcriptomic studies on G. Lacustrine goby ( Gobiopterus lacustris ) belongs to a genus of gobies that are small in size and endemic to freshwater, brackish waters or coastal environments around the Indian and Pacific oceans.

Custom qpcr and dpcr pbes microsynth taqman probes